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黄晋

发布于:2018-01-08 星期一 22:37:00 点击数:6681

理学博士,教授,博士生/硕士生导师

E-mail: jinhuang@hnu.edu.cn

基本信息

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黄晋,男,湖南永州人,2005年于湖南大学获理学学士学位,2009年获国家留学基金(CSC)资助赴美国佛罗里达大学进行博士联合培养(导师:谭蔚泓),2011年于湖南大学获理学博士学位(导师:王柯敏),博士毕业后留校开展博士后研究,2012年任助理教授,2015年晋升副教授,2019年晋升为教授。获得多项国家和省部级科研项目资助,主要致力于发展具有抗复杂环境干扰能力和高灵敏的荧光核酸分子探针以及在生物分析和医学上的应用研究,在信号放大、分子探针设计、细胞原位分析以及疾病早期诊断等方面取得了一系列研究成果。迄今在国际学术期刊上发表论文90余篇,论文被引用3000多次。

教育背景

2009-2011:美国佛罗里达大学 联合培养博士

2005-2011:湖南大学化学化工学院 理学博士

2001-2005:湖南大学化学化工学院 理学学士

工作履历

2019-至今:湖南大学化学化工学院 教授

2015-2019:湖南大学化学化工学院 副教授

2012-2015:湖南大学化学化工学院 助理教授

2011-2017:湖南大学化学化工学院 博士后


学术兼职

1. 湖南省化学化工学会第十二届理事会理事(2019-2023)

研究领域

生物分析化学/荧光核酸探针

科研项目

主持

国家自然科学基金面上项目(21874036)

国家自然科学基金青年项目(21205032

湖南省自然科学基金面上项目(2017JJ2039

湖南省自然科学基金青年项目(13JJ4032

国家博士后面上项目(2013M531779

生物反应器工程国家重点实验室开放课题基金项目(2014年)

化学生物传感与计量学国家重点实验室优秀青年人才培养计划项目(2019年)

化学生物传感与计量学国家重点实验室交叉学科前沿研究项目(2020年)

湖南大学优秀青年人才培育计划项目(2018年)

普通高校青年教师成长计划项目


参与

国家自然科学基金重大项目(21190044

国家自然科学基金重点项目(21735002)

学术成果

以第一作者或通讯作者身份在国际学术期刊上发表研究论文列表(按时间倒序排列如下):


2020年:

[46] Jing Li, Jiaoli Wang, Shiyuan Liu, Nuli Xie, Ke Quan, Yanjing Yang, Xiaohai Yang, Jin Huang*, Kemin Wang*. Amplified FRET nanoflare: An endogenous mRNA-powered nanomachine for intracellular microRNA imaging. Angewandte Chemie International Edition, doi.org/10.1002/anie.202008245

[45] Zhenwei Tang, Jin Huang, Hailun He, Changbei Ma*, Kemin Wang*. Contributing to liquid biopsy: optical and electrochemical methods in cancer biomarker analysis. Coordination Chemistry Reviews, 2020, 415, 213317.

[44] Nuli Xie, Haiyan Wang, Ke Quan, Feng Feng*, Jin Huang*, Kemin Wang*. Self-assembled DNA-based geometric polyhedrons: construction and applications. Trends in Analytical Chemistry, 2020, 126, 115844.

[43] Ke Quan, Cuiping Yi, Xiaohai Yang, Xiaoxiao He, Jin Huang*, Kemin Wang*. FRET-based nucleic acid probes: basic designs and applications in bioimaging. Trends in Analytical Chemistry, 2020, 124, 115784.

[42] Jiaoli Wang, Jing Li, Shiyuan Liu, Xiangxian Meng, Xiaohai Yang, Jin Huang*, Kemin Wang*. An amplified AND logic platform for cell identification.  Chemical Communications, doi.org/10.1039/D0CC04159A.

[41] Yanan Wu, Jing Li, Ke Quan, Xiangxian Meng, Qiuping Guo, Xiaohai Yang, Jin Huang*, Kemin Wang*. DNAzyme cascade for amplified detection of intracellular miRNA. Chemical Communications, doi.org/10.1039/D0CC02847A.

[40] Anmin Wang, Qing Lin, Shiyuan Liu, Jing Li, Jiaoli Wang, Ke Quan, Xiaohai Yang, Jin Huang*, Kemin Wang. Aptamer-tethered self-assembled FRET-flares for micrRNA imaging in living cancer cells. Chemical Communications, 2020, 56, 2463-2466.

[39] Qing Lin, Anmin Wang, Shiyuan Liu, Jing Li, Jiaoli Wang, Ke Quan, Xiaohai Yang, Jin Huang*, Kemin Wang. A DNA-tetrahedron-based molecular computation device for logic sensing of dual microRNAs in living cells. Chemical Communications, DOI: 10.1039/docc01231A. 

[38] Jing Li, Shijun Cai, Bing Zhou, Xiangxian Meng, Qiuping Guo, Xiaohai Yang, Jin Huang*, Kemin Wang*. Photocaged FRET nanoflares for intracelluar microRNA imaging. Chemical Communications, 2020, 56, 6126-6129.  

[37] Jing Li, Ke Quan, Yanjing Yang, Xiaohai Yang, Xiangxian Meng, Jin Huang*, Kemin Wang*. Engineering DNAzyme cascade for signal transduction and amplification. Analyst, 2020, 145, 1925-1932.


2010-2019年:

[36] Nuli Xie, Shiyuan Liu, Hongmei Fang, Yanjing Yang, Ke Quan, Jing Li, Xiaohai Yang, Kemin Wang, Jin Huang*. Three-dimensional molecular transfer from DNA nanocages to inner gold nanoparticle surfaces. ACS Nano, 2019, 13, 4174-4182. 

[35] Ke Quan, Jing Li, Jiaoli Wang, Nuli Xie, Qiaomei Wei, Jinlu Tang, Xiaohai Yang, Kemin Wang, Jin Huang*. Dual-microRNA-controlled double-amplified cascaded logic circuits for accurate discrimination of cell subtypes. Chemical Science, 2019, 10, 1442-1449.

[34]  Lin Lan, Qiuping Guo, Hemin Nie, Chang Zhou, Qingyun Cai, Jin Huang*, Xiangxian Meng*. Linear-hairpin variable primer RT-qPCR for microRNA. Chemical Science, 2019, 10, 2034-2043.

[33] Yanjing Yang, Shian Zhong, Kemin Wang, Jin Huang*. Gold nanoparticle based fluorescent oligonucleotide probes for imaging and therapy in living systems. Analyst, 2019, 144, 1052-1072.

[32] Qiaomei Wei, Jin Huang*,Jing Li, Jiaoli Wang, Xiaohai Yang, Jianbo Liu, Kemin Wang. DNA nanowire based localized catalytic hairpin assembly reaction for microRNA imaging in live cells. Chemical Science, 2018, 9, 7802-7808.

[31] Jiaoli Wang, Jin Huang*, Ke Quan, Jing Li, Yanan Wu, Qiaomei Wei, Xiaohai Yang, Kemin Wang*. Hairpin-fuelled catalytic nanobeacons for amplified microRNA imaging in live cells. Chemical Communications, 2018, 54, 10336-10339.

[30] Min Ou, Jin Huang*, Xiaohai Yang, Xiaoxiao He, Ke Quan, Yanjing Yang, Nuli Xie, Jing Li, Kemin Wang*. Live cell microRNA imaging via MnO2 nanosheets mediated “DD-A” hybridization chain reaction. ChemBioChem, 2018, 19, 147-152.

[29] Jing Li, Jin Huang*, Xiaohai Yang, Yanjing Yang, Ke Quan, Nuli Xie, Yanan Wu, Changbei Ma, Kemin Wang*. Two-color-based nanoflares for multiplexed microRNAs imaging in live cells. Nanotheranostics, 2018, 2, 96-105.

[28] Jing Li, Jin Huang*, Xiaohai Yang, Yanjing Yang, Ke Quan, Nuli Xie, Yanan Wu, Changbei Ma, Kemin Wang*. Gold nanoparticle-based 2’-O-methyl modified DNA probes for breast cancerous theranostics. Talanta, 2018, 183, 11-17.

[27] Min Ou, Jin Huang*, Xiaohai Yang, Ke Quan, Yanjing Yang, Nuli Xie, Kemin Wang*. MnOnanosheets mediated “DD-A” FRET binary probes for sensitive detection of intracellular mRNA. Chemical Science, 2017, 8, 668-672.

[26] Yangjin Yang, Jin Huang*, Xiaohai Yang, Xiaoxiao He, Ke Quan, Nuli Xie, Min Ou, Kemin Wang*. Gold nanoparticle based hairpin-locked-DNAzyme probe for amplified miRNA imaging in living cells. Analytical Chemistry, 2017, 89, 5850-5856.

[25] Yanan Wu, Jin Huang*, Xiaohai Yang, Yanjing Yang, Ke Quan, Nuli Xie, Jing Li, Changbei Ma, Kemin Wang*. Gold nanoparticle loaded split-DNAzyme probe for amplified miRNA detection in living cells. Analytical Chemistry, 2017, 89, 8377-8383.

[24] Nuli Xie, Jin Huang*, Xiaohai Yang, Xiaoxiao He, Jianbo Liu, Jiaqi Huang, Hongmei Fang, Kemin Wang*. Scallop-inspired DNA nanomachine: A ratiometric nanothermometer for intracellular temperature sensing. Analytical Chemistry, 2017, 89, 12115-12122. 

[23] Nuli Xie, Shiyuan Liu, Xiaohai Yang, Xiaoxiao He, Jin Huang*, Kemin Wang*. DNA tetrahedron nanostructures for biological applications: biosensors and drug delivery. Analyst, 2017, 142, 3322-3332.

[22] Jin Huang, He Wang, Xiaohai Yang, Ke Quan, Yanjing Yang, Le Ying, Nuli Xie, Min Ou, Kemin Wang*. Fluorescence resonance energy transfer-based hybridization chain reaction for in situ visualization of tumor-related mRNA. Chemical Science, 2016, 7, 3829-3835. 

[21] Ke Quan, Jin Huang*, Xiaohai Yang, Yanjing Yang, Le Ying, He Wang, Nuli Xie, Min Ou, Kemin Wang*. Powerful amplification cascades of FRET-based two-layer nonenzymatic nucleic acid circuits. Analytical Chemistry, 2016, 88, 5857-5864. 

[20] Yanjing Yang, Jin Huang*, Xiaohai Yang, Ke Quan, He Wang, Le Ying, Nuli Xie, Min Ou, Kemin Wang*. Aptazyme-gold nanoparticle sensor for amplified molecular probing in living cells. Analytical Chemistry, 2016, 88, 5981-5987. 

[19] Yanjing Yang, Jin Huang*, Xiaohai Yang, Ke Quan, Nuli Xie, Min Ou, Jinglu Tang, Kemin Wang*. Aptamer-based FRET nanoflares for imaging potassium ions in living cells. Chemical Communications, 2016, 52, 11386-11389. 

[18] Jin Huang, He Wang, Xiaohai Yang, Yanjing Yang, Ke Quan, Le Ying, Nuli Xie, Min Ou, Kemin Wang*. A supersandwich fluorescence in situ hybridization strategy for highly sensitive and selective mRNA imaging in tumor cells. Chemical Communications, 2016, 52, 370-373. 

[17] Nuli Xie, Jin Huang*, Xiaohai Yang, Yanjing Yang, Ke Quan, He Wang, Le Ying, Min Ou, Kemin Wang*. DNA tetrahedron-based molecular beacon for tumor-related mRNA detection in living cells. Chemical Communications, 2016, 52, 2346-2349. 

[16] Le Ying, Nuli Xie, Yanjing Yang, Xiaohai Yang, Qifeng Zhou, Bincheng Yin, Jin Huang*, Kemin Wang*. A cell-surface-anchored ratiometric i-motif sensor for extracellular pH detection. Chemical Communications, 2016, 52, 7818-7821. 

[15] Nuli Xie, Jin Huang*, Xiaohai Yang, Yanjing Yang, Ke Quan, Min Ou, Hongmei Fang, Kemin Wang*. Competition-mediated FRET-switching DNA tetrahedron molecular beacon for intracellular molecular detection. ACSsensors, 2016, 1, 1445-1452.

[14] Yanjing Yang+, Jin Huang+, Xiaohai Yang, Ke Quan, He Wang, Le Ying, Nuli Xie, Min Ou, Kemin Wang*. FRET nanoflares for intracellular mRNA detection: avoiding false positive signals and minimizing effects of system fluctuations. Journal of the American Chemical Society, 2015,137, 8340-8343. 

[13] Ke Quan+, Jin Huang+, Xiaohai Yang, Yanjing Yang, Le Ying, He Wang, Yong He, Kemin Wang*. An enzyme-free and amplified colorimetric detection strategy via target-aptamer binding triggered catalyzed hairpin assembly. Chemical Communications, 2015, 51, 937-940.

[12] Ke Quan+, Jin Huang+,  Xiaohai Yang, Yanjing Yang, Le Ying, He Wang, Yong He, Kemin Wang*. An enzyme-free and amplified colorimetric detection strategy: assembly of gold nanoparticles through target-catalytic circuits. Analyst, 2015, 140, 1004-1007.

[11]  Jin Huang, Le Ying, Xiaohai Yang, Yanjing Yang, Ke Quan, He Wang, Nuli Xie, Min Ou, Qifeng Zhou, Kemin Wang*. Ratiometric fluorescent sensing of pH values in living cells by dual-fluorophore-labeled i-motif nanoprobes. Analytical Chemistry, 2015, 87, 8724-8731. 

[10] Jin Huang, Xiaohai Yang, Xiaoxiao He, Kemin Wang*, Jianbo Liu, Hui Shi, Qing Wang, Qiuping Guo, Dinggeng He. Design and bioanalytical applications of DNA hairpin-based fluorescent probes. Trends in Analytical Chemistry, 2014, 53, 11-20.

[9] Jin Huang, Yong He, Xiaohai Yang, Kemin Wang*, Le Ying, Ke Quan, Yanjing Yang, Bincheng Yin. I-motif-based nano-flares for sensing pH changes in live cells. Chemical Communications, 2014, 50, 15768-15771.

[8] Jin Huang, Yong He, Xiaohai Yang, Kemin Wang*, Ke Quan, Xiaoping Lin. Splitaptazyme-based molecular beacons for amplified detection of adenosine. Analyst, 2014, 139, 2994-2997.

[7] Jin Huang, Yong He, Xiaohai Yang, Ke Quan, Kemin Wang*. Inhibited aptazyme-based catalytic molecular beacon for amplified detection of adenosine. Chinese Chemical Letters, 2014, 25, 1211-1214.

[6] Jin Huang, Xiaohai Yang, Xiaoxiao He, Kemin Wang*, Yong He, Ke Quan. A new strategy for designing graphene oxide-based DNA hairpin probe: fluorescence upon switching the orientation of sticky end. Chemical Communications, 2013, 49, 9827-9829.

[5] Kemin Wang*, Jin Huang, Xiaohai Yang, Xiaoxiao He, Jianbo Liu. Recent advances in fluorescent nucleic acid probes for living cell studies. Analyst, 2013, 138, 62-71.

[4] Jin Huang, Yanrong Wu, Yan Chen, Zhi Zhu, Xiaohai Yang, Chaoyong James Yang*, Kemin Wang*, Weihong Tan*. Pyrene-excimer probes based on the hybridization chain reaction for the detection of nucleic acids in complex biological fluids. Angewandte Chemie International Edition, 2011, 50, 401-404. 

[3] Jin Huang, Yan Chen, Liu Yang, Zhi Zhu, Guizhi Zhu, Xiaohai Yang, Kemin Wang*, Weihong Tan*. Amplified detection of cocaine based on strand displacement polymerization reaction and fluorescence resonance energy transfer. Biosensors and Bioelectronics, 2011, 28, 450-453.

[2] Xiaohai Yang+, Jin Huang+, Kemin Wang*, wei Li, Liang Cui, Xiaoping Li. Angiogenin-mediated photosensitizer-aptamer conjugate for photodynamic therapy. ChemMedChem, 2011, 6, 1778-1780.

[1] Jin Huang, Zhi Zhu, Suwussa Bamrungsap, Guizhi Zhu, Mingxu You, Xiaoxiao He, Keming Wang*, Weihong Tan*. Competition-mediated pyrene-switching aptasensor: probing lysozyme in human serum with a monomer-excimer fluorescence switch. Analytical Chemistry, 2010, 82, 10158-10163.

奖励与荣誉

一、指导学生毕业论文获奖情况:

1. 指导应乐的毕业论文《基于荧光共振能量转移的i-motif探针用于细胞内外PH值检测》被评为湖南省优秀硕士毕业论文(2018)。

2. 指导欧敏的毕业论文《MnO2纳米片介导的“DD-A”FRET核酸探针用于细胞内RNA检测》被评为湖南省优秀硕士毕业论文(2019)。

3. 指导杨艳婧的毕业论文《基于金纳米颗粒的荧光核酸探针用于细胞内传感》被评为湖南省优秀博士毕业论文(2019)。


二、指导学生获奖学金情况:

2015年:博士生杨艳婧获国家奖学金和校奖学金;博士生全珂获校奖学金;硕士生应乐获国家奖学金。

2016年:博士生全珂获国家奖学金和校奖学金;博士生谢努力获校奖学金;硕士生欧敏获国家奖学金。

2017年:博士生杨艳婧获校奖学金;硕士生吴亚南获国家奖学金。

2018年:博士生谢努力获国家奖学金;硕士生韦巧梅获国家奖学金。